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Hoefer te77x semi-dry transfer unit
Te77x Semi Dry Transfer Unit, supplied by Hoefer, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/te77x+semi-dry+transfer+unit/semi+dry+transfer+te70x/pmc11706503-137-16-20
Average 90 stars, based on 1 article reviews
te77x semi-dry transfer unit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polyacrylamide Gel Electrophoresis:

Article Title: Reciprocal regulation of chromatin state and architecture by HOTAIRM1 contributes to temporal collinear HOXA gene activation
Article Snippet: Protein samples from cell fractionation and RNA immunoprecipitation (RIP) analysis were denatured in SDS sample buffer (62.5 mM Tris–HCl pH 6.8, 2% SDS, 7.5% glycerol, 5% β-mercaptoethanol, 0.04% bromophenol blue) for 10 min at 95°C, resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane (Whatman Protran ® ) using a Hoefer TE77X semi-dry transfer unit at 20 V for 45 min. Immunoblotting was performed with antibodies against Hsp90 (Santa Cruz (at-115), gift from Dr S. Huang, McGill), Lamin A/C (Santa Cruz, sc-20681), UTX (Bethyl Laboratories, A302-374A), SUZ12 (Abcam, ab12073) and ASH2L (Bethyl Laboratories, A300-489A).

Article Title: Evidence of avian and human influenza A virus infection in farmed Siamese crocodiles ( Crocodylus siamensis ) in Thailand
Article Snippet: The electrophoresed proteins in gel were then blotted onto a nitrocellulose membrane (Bio-Rad, Hercules, CA) using TE77X semi-dry transfer unit (Hoefer Inc).

Article Title: A conserved HOTAIRM1-HOXA1 regulatory axis contributes early to neuronal differentiation
Article Snippet: Protein samples from RIP (~100,000 cells or 1%) or RNAi experiments (~150,000 cells) were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo GibcoTM) with a Hoefer TE77X semi-dry transfer unit at 20 V for 1 hour.

Article Title: Methods for making and using modified oocytes
Article Snippet: Separated samples were electroblotted onto Nytran SuPerCharge TurboBlotter membrane (Whatman, Sanford, Me.) using a TE77X semi-dry transfer unit (Hoefer, Holliston, Mass.) and immobilized by ultraviolet cross-linking.

Article Title: Homozygous missense mutation in UQCRC2 associated with severe encephalomyopathy, mitochondrial complex III assembly defect and activation of mitochondrial protein quality control.
Article Snippet: The mitochondrial respiratory chain (MRC) complex III (CIII) associates with complexes I and IV (CI and CIV) into supercomplexes.. We identified a novel homozygous missense mutation (c.665G>C; p.Gly222Ala) in UQCRC2 coding for structural subunit Core 2 in a patient with severe encephalomyopathy.. The structural data suggest that the Gly222Ala exchange might result in an altered spatial arrangement in part of the UQCRC2 subunit, which could impact specific protein-protein interactions.

Transferring:

Article Title: Reciprocal regulation of chromatin state and architecture by HOTAIRM1 contributes to temporal collinear HOXA gene activation
Article Snippet: Protein samples from cell fractionation and RNA immunoprecipitation (RIP) analysis were denatured in SDS sample buffer (62.5 mM Tris–HCl pH 6.8, 2% SDS, 7.5% glycerol, 5% β-mercaptoethanol, 0.04% bromophenol blue) for 10 min at 95°C, resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane (Whatman Protran ® ) using a Hoefer TE77X semi-dry transfer unit at 20 V for 45 min. Immunoblotting was performed with antibodies against Hsp90 (Santa Cruz (at-115), gift from Dr S. Huang, McGill), Lamin A/C (Santa Cruz, sc-20681), UTX (Bethyl Laboratories, A302-374A), SUZ12 (Abcam, ab12073) and ASH2L (Bethyl Laboratories, A300-489A).

Article Title: Evidence of avian and human influenza A virus infection in farmed Siamese crocodiles ( Crocodylus siamensis ) in Thailand
Article Snippet: The electrophoresed proteins in gel were then blotted onto a nitrocellulose membrane (Bio-Rad, Hercules, CA) using TE77X semi-dry transfer unit (Hoefer Inc).

Article Title: A conserved HOTAIRM1-HOXA1 regulatory axis contributes early to neuronal differentiation
Article Snippet: Protein samples from RIP (~100,000 cells or 1%) or RNAi experiments (~150,000 cells) were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo GibcoTM) with a Hoefer TE77X semi-dry transfer unit at 20 V for 1 hour.

Article Title: Methods for making and using modified oocytes
Article Snippet: Separated samples were electroblotted onto Nytran SuPerCharge TurboBlotter membrane (Whatman, Sanford, Me.) using a TE77X semi-dry transfer unit (Hoefer, Holliston, Mass.) and immobilized by ultraviolet cross-linking.

Article Title: Homozygous missense mutation in UQCRC2 associated with severe encephalomyopathy, mitochondrial complex III assembly defect and activation of mitochondrial protein quality control.
Article Snippet: The mitochondrial respiratory chain (MRC) complex III (CIII) associates with complexes I and IV (CI and CIV) into supercomplexes.. We identified a novel homozygous missense mutation (c.665G>C; p.Gly222Ala) in UQCRC2 coding for structural subunit Core 2 in a patient with severe encephalomyopathy.. The structural data suggest that the Gly222Ala exchange might result in an altered spatial arrangement in part of the UQCRC2 subunit, which could impact specific protein-protein interactions.

Membrane:

Article Title: Reciprocal regulation of chromatin state and architecture by HOTAIRM1 contributes to temporal collinear HOXA gene activation
Article Snippet: Protein samples from cell fractionation and RNA immunoprecipitation (RIP) analysis were denatured in SDS sample buffer (62.5 mM Tris–HCl pH 6.8, 2% SDS, 7.5% glycerol, 5% β-mercaptoethanol, 0.04% bromophenol blue) for 10 min at 95°C, resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane (Whatman Protran ® ) using a Hoefer TE77X semi-dry transfer unit at 20 V for 45 min. Immunoblotting was performed with antibodies against Hsp90 (Santa Cruz (at-115), gift from Dr S. Huang, McGill), Lamin A/C (Santa Cruz, sc-20681), UTX (Bethyl Laboratories, A302-374A), SUZ12 (Abcam, ab12073) and ASH2L (Bethyl Laboratories, A300-489A).

Article Title: Evidence of avian and human influenza A virus infection in farmed Siamese crocodiles ( Crocodylus siamensis ) in Thailand
Article Snippet: The electrophoresed proteins in gel were then blotted onto a nitrocellulose membrane (Bio-Rad, Hercules, CA) using TE77X semi-dry transfer unit (Hoefer Inc).

Article Title: A conserved HOTAIRM1-HOXA1 regulatory axis contributes early to neuronal differentiation
Article Snippet: Protein samples from RIP (~100,000 cells or 1%) or RNAi experiments (~150,000 cells) were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo GibcoTM) with a Hoefer TE77X semi-dry transfer unit at 20 V for 1 hour.

Article Title: Methods for making and using modified oocytes
Article Snippet: Separated samples were electroblotted onto Nytran SuPerCharge TurboBlotter membrane (Whatman, Sanford, Me.) using a TE77X semi-dry transfer unit (Hoefer, Holliston, Mass.) and immobilized by ultraviolet cross-linking.

Article Title: Homozygous missense mutation in UQCRC2 associated with severe encephalomyopathy, mitochondrial complex III assembly defect and activation of mitochondrial protein quality control.
Article Snippet: The mitochondrial respiratory chain (MRC) complex III (CIII) associates with complexes I and IV (CI and CIV) into supercomplexes.. We identified a novel homozygous missense mutation (c.665G>C; p.Gly222Ala) in UQCRC2 coding for structural subunit Core 2 in a patient with severe encephalomyopathy.. The structural data suggest that the Gly222Ala exchange might result in an altered spatial arrangement in part of the UQCRC2 subunit, which could impact specific protein-protein interactions.

Cell Fractionation:

Article Title: Reciprocal regulation of chromatin state and architecture by HOTAIRM1 contributes to temporal collinear HOXA gene activation
Article Snippet: Protein samples from cell fractionation and RNA immunoprecipitation (RIP) analysis were denatured in SDS sample buffer (62.5 mM Tris–HCl pH 6.8, 2% SDS, 7.5% glycerol, 5% β-mercaptoethanol, 0.04% bromophenol blue) for 10 min at 95°C, resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane (Whatman Protran ® ) using a Hoefer TE77X semi-dry transfer unit at 20 V for 45 min. Immunoblotting was performed with antibodies against Hsp90 (Santa Cruz (at-115), gift from Dr S. Huang, McGill), Lamin A/C (Santa Cruz, sc-20681), UTX (Bethyl Laboratories, A302-374A), SUZ12 (Abcam, ab12073) and ASH2L (Bethyl Laboratories, A300-489A).

Article Title: Evidence of avian and human influenza A virus infection in farmed Siamese crocodiles ( Crocodylus siamensis ) in Thailand
Article Snippet: The electrophoresed proteins in gel were then blotted onto a nitrocellulose membrane (Bio-Rad, Hercules, CA) using TE77X semi-dry transfer unit (Hoefer Inc).

Article Title: A conserved HOTAIRM1-HOXA1 regulatory axis contributes early to neuronal differentiation
Article Snippet: Protein samples from RIP (~100,000 cells or 1%) or RNAi experiments (~150,000 cells) were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo GibcoTM) with a Hoefer TE77X semi-dry transfer unit at 20 V for 1 hour.

Article Title: Methods for making and using modified oocytes
Article Snippet: Separated samples were electroblotted onto Nytran SuPerCharge TurboBlotter membrane (Whatman, Sanford, Me.) using a TE77X semi-dry transfer unit (Hoefer, Holliston, Mass.) and immobilized by ultraviolet cross-linking.

Article Title: Homozygous missense mutation in UQCRC2 associated with severe encephalomyopathy, mitochondrial complex III assembly defect and activation of mitochondrial protein quality control.
Article Snippet: The mitochondrial respiratory chain (MRC) complex III (CIII) associates with complexes I and IV (CI and CIV) into supercomplexes.. We identified a novel homozygous missense mutation (c.665G>C; p.Gly222Ala) in UQCRC2 coding for structural subunit Core 2 in a patient with severe encephalomyopathy.. The structural data suggest that the Gly222Ala exchange might result in an altered spatial arrangement in part of the UQCRC2 subunit, which could impact specific protein-protein interactions.

Immunoprecipitation:

Article Title: Reciprocal regulation of chromatin state and architecture by HOTAIRM1 contributes to temporal collinear HOXA gene activation
Article Snippet: Protein samples from cell fractionation and RNA immunoprecipitation (RIP) analysis were denatured in SDS sample buffer (62.5 mM Tris–HCl pH 6.8, 2% SDS, 7.5% glycerol, 5% β-mercaptoethanol, 0.04% bromophenol blue) for 10 min at 95°C, resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane (Whatman Protran ® ) using a Hoefer TE77X semi-dry transfer unit at 20 V for 45 min. Immunoblotting was performed with antibodies against Hsp90 (Santa Cruz (at-115), gift from Dr S. Huang, McGill), Lamin A/C (Santa Cruz, sc-20681), UTX (Bethyl Laboratories, A302-374A), SUZ12 (Abcam, ab12073) and ASH2L (Bethyl Laboratories, A300-489A).

Article Title: Evidence of avian and human influenza A virus infection in farmed Siamese crocodiles ( Crocodylus siamensis ) in Thailand
Article Snippet: The electrophoresed proteins in gel were then blotted onto a nitrocellulose membrane (Bio-Rad, Hercules, CA) using TE77X semi-dry transfer unit (Hoefer Inc).

Article Title: A conserved HOTAIRM1-HOXA1 regulatory axis contributes early to neuronal differentiation
Article Snippet: Protein samples from RIP (~100,000 cells or 1%) or RNAi experiments (~150,000 cells) were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo GibcoTM) with a Hoefer TE77X semi-dry transfer unit at 20 V for 1 hour.

Article Title: Methods for making and using modified oocytes
Article Snippet: Separated samples were electroblotted onto Nytran SuPerCharge TurboBlotter membrane (Whatman, Sanford, Me.) using a TE77X semi-dry transfer unit (Hoefer, Holliston, Mass.) and immobilized by ultraviolet cross-linking.

Article Title: Homozygous missense mutation in UQCRC2 associated with severe encephalomyopathy, mitochondrial complex III assembly defect and activation of mitochondrial protein quality control.
Article Snippet: The mitochondrial respiratory chain (MRC) complex III (CIII) associates with complexes I and IV (CI and CIV) into supercomplexes.. We identified a novel homozygous missense mutation (c.665G>C; p.Gly222Ala) in UQCRC2 coding for structural subunit Core 2 in a patient with severe encephalomyopathy.. The structural data suggest that the Gly222Ala exchange might result in an altered spatial arrangement in part of the UQCRC2 subunit, which could impact specific protein-protein interactions.

Western Blot:

Article Title: Reciprocal regulation of chromatin state and architecture by HOTAIRM1 contributes to temporal collinear HOXA gene activation
Article Snippet: Protein samples from cell fractionation and RNA immunoprecipitation (RIP) analysis were denatured in SDS sample buffer (62.5 mM Tris–HCl pH 6.8, 2% SDS, 7.5% glycerol, 5% β-mercaptoethanol, 0.04% bromophenol blue) for 10 min at 95°C, resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane (Whatman Protran ® ) using a Hoefer TE77X semi-dry transfer unit at 20 V for 45 min. Immunoblotting was performed with antibodies against Hsp90 (Santa Cruz (at-115), gift from Dr S. Huang, McGill), Lamin A/C (Santa Cruz, sc-20681), UTX (Bethyl Laboratories, A302-374A), SUZ12 (Abcam, ab12073) and ASH2L (Bethyl Laboratories, A300-489A).

Article Title: Evidence of avian and human influenza A virus infection in farmed Siamese crocodiles ( Crocodylus siamensis ) in Thailand
Article Snippet: The electrophoresed proteins in gel were then blotted onto a nitrocellulose membrane (Bio-Rad, Hercules, CA) using TE77X semi-dry transfer unit (Hoefer Inc).

Article Title: A conserved HOTAIRM1-HOXA1 regulatory axis contributes early to neuronal differentiation
Article Snippet: Protein samples from RIP (~100,000 cells or 1%) or RNAi experiments (~150,000 cells) were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo GibcoTM) with a Hoefer TE77X semi-dry transfer unit at 20 V for 1 hour.

Article Title: Methods for making and using modified oocytes
Article Snippet: Separated samples were electroblotted onto Nytran SuPerCharge TurboBlotter membrane (Whatman, Sanford, Me.) using a TE77X semi-dry transfer unit (Hoefer, Holliston, Mass.) and immobilized by ultraviolet cross-linking.

Article Title: Homozygous missense mutation in UQCRC2 associated with severe encephalomyopathy, mitochondrial complex III assembly defect and activation of mitochondrial protein quality control.
Article Snippet: The mitochondrial respiratory chain (MRC) complex III (CIII) associates with complexes I and IV (CI and CIV) into supercomplexes.. We identified a novel homozygous missense mutation (c.665G>C; p.Gly222Ala) in UQCRC2 coding for structural subunit Core 2 in a patient with severe encephalomyopathy.. The structural data suggest that the Gly222Ala exchange might result in an altered spatial arrangement in part of the UQCRC2 subunit, which could impact specific protein-protein interactions.



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